Abstract: Tung oil,a natural resource was chosen as the controlled release materials to produce the controlled release fertilizer. We studied the influence of controlled release fertilizer in greenhouse soilless culture on the content of Potassium and organic acid of in tobacco. The plant maximal leaf area and height were 1 145 cm² and 80 cm respectively under the controlled compound fertilizer while that control treatment were 994 cm² and 74 cm at maturity,with differences for 151 cm² and 6 cm. The content of Potassium in upper,middle and lower leaves with controlled compound fertilizer was 0.1%,0.08%,0.08% greater than that of compound fertilization respectively,while the content of organic acid in the maximal leaf area was increased by 2.431 μg·g^-1. It proves that controlled released fertilizer can increase the quality of tobacco in greenhouse hydroponic.