Abstract: To investigate good quality and safety in oil flax(Linum usitatissimum Linn) production,field experiments were conducted in Ordos City of Inner Mongolia autonomous region,Datong City of Shanxi Province,Baiyin and Lanzhou Cities of Gansu Province.The study compared the effects of the applications of four different maures on oil flax quality characters and yield.The four organic fertilizers included farmyard manure(T2),oil flax residue(T3),Qingtiaobu(T4) and Woliheng(T5) bio-fertilizers.Treatments without fertilizer(T0) and with chemical fertilizer(T1) were used as controls.The results showed different responses in oil flax quality characters to different manures in four experiment sites.Effects of oil flax quality under four manure treatments were primarily reflected in variation of fat content and saturated fatty acid.Accumulation of oil flax quality components were influenced by organic manure types and field environmental factors,but no homogeneity appeared in this course.Compared to T0 and T1,treatments of T2,T3,T4 and T5 have influence on palmic acid,oleic acid,linoleic acid,linolenic acid and lignan content,there are no significant difference appeared in four manure treatments,therefore,major difference embodied in variety of fat content,stearic acid and iodine value.Woliheng increased fat content by 1.24% significantly over chemical fertilizer,farmyard manure and Woliheng decreased stearic acid content by 4.97% and 12.04% over fertilizer.In the four experiment sites,higher saturated fatty acid content appeared in Lanzhou and Baiyin,besides for fat content,oleic acid,linoleic acid in Ordos and linolenic acid in Lanzhou and Datong.An average high-yield up to 2 356 kg·hm^-2 and 2 067 kg·hm^-2 were majored in Ordos and Lanzhou site under the four manure treatments.As for different valuable components in manures and different soil conditions and ecological surroundings,the four manures appeared different effect on oil flax quality characters and yield,the suggestion was proposed for establishing high quality oil flax production base in Lanzhou and Ordos.