Abstract: The conservation of wetland ecological environment is of vital importance to the improvement of endangered species habitat, while the urgent task is to establish a targeted and reasonable evaluation index system for the assessment. In this paper, a wetland ecological vulnerability evaluation system in Honghe National Nature Reserve, based on Pressure-Status-Response (PSR) concept model, was built up in resting habitat, foraging habitat, landscape habitat and emergency foraging habitat of Oriental White Stork; then after, a comprehensive evaluation method was applied to evaluate the wetland ecological vulnerability of Honghe Nature Reserve in 2013. The result showed that the wetland ecological vulnerability of Honghe Nature Reserve in 2013 was in a potential vulnerability degree with a fine quality of the ecological environment, which was closely related to the implementation of governmental policy and the protection consciousness of local people.