Abstract: The livestock manure composting can effectively degrade antibiotics such as oxytetracycline.Temperature is considered as one of the important factors during composting.However, the roles of microorganisms in composting process are often neglected.Aiming to understand oxytetracycline degradation and microbial community changes, we conducted an experiment with two concentrations of oxytetracycline, 0 as a control(CK) and 60 mg·kg^-1 as a treated group(TG).The results showed that the degradation ratios of oxytetracycline were 93.3% and 96.7% at 28 days and 63 days, respectively.Also, the relative abundances for Paenibacillus, Bacillus and Thermobacillus increased from 0.04%, 0 and 0 to 4.25%, 1.16% and 1.68% in TG group, while only increased to 0.20%, 0.02% and 0.09% in CK, respectively.Moreover, the effect of Bacillus, Brevibacillus and Chelatococcus on oxytetracycline degradation was significant, indicating that microbial degradation may play an important role in degrading oxytetracycline, which confirmed a close relationship between the oxytetracycline degradation and microorganism community during cattle manure composting.