Abstract: To investigate the toxic effect of calcium-rich characteristics of karst soil on maize and the mitigation of high calcium stress by AM fungi,a greenhouse pot experiment was conducted to investigate the effects of inoculation with arbuscular mycorrhiza fungi(Glomus mosseae) on the growth,and bio-chemical characteristics of maize seedlings under different Ca levels.The results indicated that the high calcium treatment(1.0 g·kg^-1,excluding background value) had a significant inhibitory effect on the growth and development of maize,and significantly reduced the plant height,biomass,photosynthetic rate,transpiration rate,stomatal conductance and soluble sugar content of maize seedlings.The inoculation of AM fungi under low calcium treatment significantly increased the plant height and biomass,enhanced the photosynthetic rate and,significantly increased the soluble sugar content but reduced the accumulation of MDA,and thus alleviated the degree of membrane lipid peroxidation of leaves and promoted the growth and development of seedlings.However,the inoculation treatment with high calcium had no positive effect on maize seedlings,poor symbiotic relationship was established between AMF and plants,and had no significant impacts on the growth and physiological characteristics of maize seedlings.