Legumes are important cash and fodder crops in the world.China′s rigid demands for crops, such as, soybean and alfalfa, have increased rapidly in recent years, but the legumes′ yields have increased slowly.Besides legumes′variety and farming mode, their abiotic and biotic stresses seriously limit the production and yield.In this paper, we systematically summarized the response of legume tubulin to drought stress, heavy metal stress and light system regulation, discussed how legume tubulin participated in pathogen invasion and Ca²⁺ signal regulation, and introduced the cloned microtubule genes in Glycine max, Phaseolus vulgaris and Medicago truncatula; which would provide theoretical guidance for improving stress tolerance of legumes through genetic improvement of microtubule genes.