Abstract: In order to understand the effect of different biochar application rates on the metabolic characteristics of carbon sources of soil microbial community in acidified Albic soil in Northeast China, we conducted an experiment including following treatments: control(C0t), 15 t·hm^-2 biochar(C1t), 30 t·hm^-2 biochar(C2t) and 60 t·hm^-2 biochar(C3t); and analyzed their effects on the functional diversity of soil microbial community.The results showed that additional biochar application significantly increased the average well color development(AWCD) value, Shannon Wiener diversity index, richness index and Simpson index of soil microorganism compared with C0t treatment.With the increased rate of biochar application, the utilization of carbon sources changed from polymers towards amino acids and carboxylic acids.The utilization energy of D-xylose, D-galacturonic acid, N-acetyl-D-glucosamine, l-asparagic acid, l-threonine, Tween-40, Tween 80, hepatose and putrescine was significantly higher in the addition of biochar application than that of C0t treatment, respectively.The principal component analysis showed that carbohydrates and amines were the main carbon sources of carbon source utilization under different treatments, while amino acids, carboxylic acids, phenolic acids and polymers were the sensitive carbon sources to distinguish the differences between C0t treatment and the treatment with biochar application.C2t treatment was a reasonable application rate for the soil micro-ecological environment with corn continuous cropping.