Abstract: The damage of Ditylenchus destructor to potato has occurred in many places in China. In order to identify the biotype of D. destructor and its reproductive capacity on different hosts, the nematodes were isolated by Baermann funnel method and identified by molecular method according to the description of GB/T 29577-2013. The ITS sequences of D. destructor from potato and sweet potato were selected from NCBI for phylogenetic analysis. D. destructor was cultured with carrot callus and then inoculated into the roots of corn, potato, sorghum, soybean and wheat to determine its reproductive capacity. The results showed that the isolated D. destructor was type B. The genetic evolution analysis showed that D. destructor from sweet potato and potato hosts was obviously divided into two branches. It would be inferred that the evolution of D. destructor was affected by the host. D. destructor reproduced on all the tested hosts. The reproduction coefficient of D. destructor on wheat was the lowest, followed by soybean. Planting wheat and soybean may have inhibitory effect on D. destructor.